Laragen provides reliable, fast, and affordable STR-based human cell-line authentication services. Our STR cell-line authentication tests complies with ANSI/ATCC ASN-0002-2011 guidelines. Laragen has over 10 years experience working on STR analysis.
STR (Short Tandem Repeat) has been used in human paternity and forensic fields for more than 20 years. In recent years, STR-based analysis has been used as the “golden standard” in human cell-line authentication.
ANIS/ATCC ASN-0002-2011 recommends eight STR markers and one gender marker in human cell-line authentication: TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818 and Amelogenin.
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Currently Laragen is using Promega PowerPlex 16 HS and GenePrint 10 kits in human cell line authentication.
Laragen provides research and GLP grade (compliant with FDA CFR 21, Part 58) cell-line authentication and Mycoplasma detection.
The PowerPlex 16 HS system allows co-amplification and three-color detection of sixteen markers (fifteen STR markers and Amelogenin), including Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. The matching probability of the PowerPlex® 16 HS is greater than 1 in 2.92 × 1017.
The GenePrint 10 System allows co-amplification and three-color detection of ten human markers (nine STR markers, Amelogenin for gender identification), including D21S11, TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317, D5S818.. These markers collectively provide a genetic profile with a random match probability of 1 in 2.92 × 109. GenePrint is an economical choice for confirmation of cell-line identity.
Mycoplasma belongs to the family Mollicutes; including, Mycoplasma, Acholeplasma, Ureaplasma, Entomoplasma, and Spiroplasma. They are the smallest free-living and self-replicating organisms. Six Mycoplasma species: M.orale, M.arginii, M.fermentans, M.salivarum, M.hyorhinis and A.laidlawii, account for 95% of all Mycoplasma contamination in cell cultures.
Mycoplasma testing is carried out using two methods at the same time point. Detecting Mycoplasma by PCR amplification is highly sensitive and is recommended by The American Type Culture Collection (ATCC) and nucleic acid amplification technique (NAT) is now accepted by FDA as a compendial method for Mycoplasma detection.
Laragen provides two NAT Mycoplasma detection methods: gel based PCR detection and real-time PCR based detection. We confirm Mycoplasma species by 16S rRNA sequencing when we use gel based PCR method.
Both gel based PCR and real-time PCR Mycoplasma detection tests will detect a broad range of Mycoplasma under the European Pharmacopeia (EP) chapter 2.2.7 and the United States Pharmacopeia (USP) chapter 63.
Laragen provides research and GLP grade (compliant with FDA CFR 21, Part 58) Mycoplasma detection services.
We use cytochrome C oxidase I (COXI) gene to identify the species of the cell cultures.Get a Quote