Laragen provides reliable, fast, and affordable STR based human cell line authentication services. Our STR cell line authentication tests complies with ANSI/ATCC ASN-0002-2011 guidelines. Laragen has over 10 year experience working on STR PCR and fragment analysis.
STR (Short Tandem Repeat) has been used in human paternity and forensic fields for more than 20 years. In recent years, STR-based analysis has been used as the “golden standard” in human cell line authentication.
ANIS/ATCC ASN-0002-2011 recommends eight STR markers and one gender marker in human cell line authentication: TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818 and Amelogenin.
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Currently Laragen is using Promega PowerPlex 16 HS and GenePrint 10 kits in human cell line authentication. The PowerPlex 16 HS system allows co-amplification and three-color detection of sixteen markers (fifteen STR markers and Amelogenin), including Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818.
The PowerPlex 16 HS system allows co-amplification and three-color detection of sixteen markers (fifteen STR markers and Amelogenin), including Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. The matching probability of the PowerPlex® 16 HS is greater than 1 in 2.92 × 1017.
The GenePrint 10 System allows co-amplification and three-color detection of ten human markers (nine STR markers, Amelogenin for gender identification and one mouse marker), including D21S11, TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317, D5S818 and the mouse marker. These markers collectively provide a genetic profile with a random match probability of 1 in 2.92 × 109. GenePrint is able to detect any mouse contaminants down to as little as 1% in a human cell line.
Mycoplasma testing is carried out using two methods at the same time point. Detecting mycoplasma by PCR amplification is highly sensitive and is recommended by The American Type Culture Collection (ATCC.)
All contaminated cultures should be treated or discarded. Mycoplasma belongs to the family Mollicutes; including Mycoplasma, Acholeplasma, Ureaplasma, Entomoplasma, and Spiroplasma. They are the smallest free-living and self-replicating organisms. Six Mycoplasma species: M.orale, M.arginii, M.fermentans, M.salivarum, M.hyorhinis and A.laidlawii, account for 95% of all mycoplasma contamination in cell cultures.
We use cytochrome C oxidase I (COXI) gene to identify the species of the cell cultures. Our species specific primers identify the following species:
We will also sequence the COXI gene if the species of the cell culture is not in the list and identify the species based on the COXI sequences.Get a Quote