We will provide free repeats to troubleshoot failed reactions. If a large number of reactions failed, we will repeat a few samples to troubleshoot. If the repeated reactions work, we can repeat all failed reactions at a charge upon request.
After your sequences are ready, you will receive a notification email. You can login into your account here to download your sequences.
It depends on the quality of the DNA templates and the sequences. You can get > 900 bp per read on average. If you want to get more than 900 bp, please contact us and we will reanalyze the sequence for you.
It depends on the quality of the DNA templates. You can get reliable sequences starting from 17 - 25 bp away from the primer.
You can ship your DNA sample either in water or EB buffer (No EDTA) by US mail or FedEx (preferred) at room temperature to:
10601 Virginia Ave
Culver City, CA 90232
We also provide free pick-up in the Los Angeles Metro area.
|Type of sample||Turnaround Time|
|Sequencing ready-to-run sample||Overnight|
|Sequencing premix sample||Overnight|
|Sequencing purified plasmid and PCR sample||Overnight|
|Sequencing un-purified PCR sample||Overnight|
|Bacterial cell (RCA)||24 to 48 hours|
|Genotyping animal tissue with gel-based or qPCR||48-72 hours|
|Genotyping animal gDNA with gel-based or qPCR||48-72 hours|
|Fragment ready-to-run analysis||Overnight|
|Microbial ID coloines or cell pellet||5 to 7 days|
|Genotyping copy number analysis||72 hours|
|Cell line authentication||3-5 days|
|Standard Primers Sequences|
|M13 Forward||GTA AAA CGA CGG CCA GT|
|M13 Reverse||CAG GAA ACA GCT ATG AC|
|T7 Promoter||TAA TAC GAC TCA CTA TAG GG|
|T3||ATT AAC CCT CAC TAA AG|
|SP6||ATT TAG GTG ACA CTA TAG|
|T7 Terminator||CTA GTT ATT GCT CAG CGG TG|
|pGEX 5'||GGG CTG GCA AGC CAC GTT TGG TG|
|pGEX 3'||CCG GGA GCT GCA TGT GTC AGA GG|
|RV3||CTA GCA AAA TAG GCT GTC CCC|
|BGH Reverse||TAG AAG GCA CAG TCG AGG C|
|pBADfor||ATG CCA TAG CTT TTT ATC C|
|pBADrev||GAT TTA ATC TGT ATC AGG|
|pFastBacFwd||TAT TCC GGA TTA TTC ATA CCG TC|
|pFastBacRev||GTA TGG CTG ATT ATG ATC CTC|
|CMVfor||CGC AAA TGG GCG GTA GGC GTG|
|CMVRev||AGT AGG AAA GTC CCG TAA GG|
Unless you specifically request, we normally keep our clients' DNA samples and primers for three weeks. The DNA samples and primers will be trashed after three weeks.
We use a fluorometer coupled with Hoechst 33342 dye to measure DNA concentration. Because Hoechst 33342 only binds to double strand DNA, not protein, RNA or other contaminants, the DNA concentration measured with this technique is more accurately measured. In comparison, nanodrop is based on absorption peak at 260/280 nm wavelength. Many contaminants in the DNA solution could affect the OD reading. We found if the DNA concentration measured by fluorometer is significantly lower than the client's concentration measured by spectrophotometer (such as nanodrop) the sequencing reactions tends to fail due to the contaminants present in the DNA samples.
Sequence Scanner is a powerful software to view AB trace files. Sequencing Scanner is able to provide a variety of QC data and is able to view multiple trace files.
Chromas 2.6.4 is a PC based freeware to view AB trace files.
4Peaks V1.8 is a Mac OSX 10.7 or above based freeware to view AB trace files.
qPCR Ready-To-Run Request Form
Fragment Analysis Request Form
Trace file sample to demonstrate long sequencing reads
All new users will need to create a login account on our DNALIMS server here. For other services, please email a copy of the corresponding request form.
Sequencing clients will receive an automated email notification when the sequences are ready to download. You can enter more than one email address in the email field separated by commas.
After you receive the email notification, you can login to your DNALIMS account and download your sequences
For all other services, you will receive emails with your results attached in Excel format. The raw data, such as .fsa files, are available to download from dropbox link we send.
We recommend you use either Sequence Scanner or Chromas 2.6.4(PC-Based) and 4Peaks V1.8(OSX 10.7+) to view electropherograph file (.ab1). We set our sequencing read length at 1000 bp. If the sequences are good and you would like to have longer reads, you can submit the .ab1 files to PeakTrace.