Laragen is the first commercial company to apply real-time PCR technique in transgenic genotyping. In 2001, Laragen successfully applied Taqman technique to genotype APP and PS1 double transgenic mice.
Laragen's unique knowledge and experience with Mouse Genotyping stands us apart from others. Our outstanding service with standard turnaround times of just 48 hours, combined with special same-day service upon request, ensures you will get your results when you need them.
Laragen developed a proprietary high throughput DNA extraction technique to extract high quality genomic DNA from mouse/rat tissues. High quality genomic DNA, robust PCR assays and stringent QC makes our mouse genotyping results very accurate (>99.95%).
For sample preparation, we require 0.3 to 0.4 cm tail clips and 2 mm ear punches. It is important that the tissue samples have similar sizes. Samples can be sent in either 1.7 ml Eppendorf tubes or 1.1ml 8-strip microtubes in 96-well boxes.
Samples in 96-well plate format should be arranged by the columns as indicated below:
Depending on the number of samples and nature of the tests, we either waive the initial set-up fee or only charge a nominal set-up fee to cover the reagent cost. For the mouse models that are not in the Laragen test pool, we need to have the following to develop and convert to the Laragen mouse genotyping protocols:
Mouse tails should be send on dry ice and genomic DNA samples can be sent at room temperature to
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Most of Laragen’s mouse genotyping tests are probe-based TaqMan assays. Laragen’s TaqMan assays have an internal positive control on a housekeeping gene to ensure the test integrity. Also, all tests have CAR(carrier), HOMO and HET(if applicable), WT(wildtype) and NTC controls. The samples in the grey zone will be repeated multiple times to ensure test accuracy, free of charge.
A small portion of Laragen’s mouse genotyping tests are gel-based PCR assays. Like probe-based assays, we also include an internal positive control PCR on a house-keeping gene to ensure PCR integrity. All PCR reactions are optimized to yield accurate genotyping results even at suboptimal conditions. A vigorous SOP is implemented to eliminate PCR contamination problems.
Laragen also provides heterozygosity tests for transgenic mice-based on qPCR (TaqMan) via copy number analysis. Normalized high quality genomic DNA samples are tested in triplicates and the transgene copy number of unknown samples are compared with the known het and homo controls.
Laragen offers low to medium throughput SNP genotyping by the following two platforms:
Laragen has more than 10 years experience on trinucleotide repeat sizing analysis from various mouse models: